- Backbone size (bp) 5056
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Bacterial expression plasmid for GST tagged with a PKA phosphorylation site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGSTag was a gift from David Ron (Addgene plasmid # 21877)
For your References section:pGSTag--a versatile bacterial expression plasmid for enzymatic labeling of recombinant proteins. Ron D, Dressler H. Biotechniques. 1992 Dec . 13(6):866-9. PubMed 1476738
Map generated by Addgene from full sequence supplied by depositor.