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pFR_HCV_xb
(Plasmid #11510)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11510 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRL-TK
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4045
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site see comments (unknown if destroyed)
  • 3′ cloning site see comments (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The plasmid constructed by PCR cloning HCV IRES into the NheI site of pRL-TKx6 and inserting the Firefly luciferase ORF from pGL3 into the resulting vectorl.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFR_HCV_xb was a gift from Phil Sharp (Addgene plasmid # 11510 ; http://n2t.net/addgene:11510 ; RRID:Addgene_11510)
  • For your References section:

    Short RNAs Repress Translation after Initiation in Mammalian Cells. Petersen CP, Bordeleau ME, Pelletier J, Sharp PA. Mol Cell. 2006 Feb 17. 21(4):533-42. 10.1016/j.molcel.2006.01.031 PubMed 16483934