Addgene - H2B-GFP Plasmid Data

Plasmid 11680: H2B-GFP

Gene/insert name:
Human histone H2B
Insert size:
380
Species:
H. sapiens (human)
GenBank ID:
X00088
Entrez Gene:
HIST1H2BJ (H2B/r, H2BFR, H2BJ)
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N1
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4733
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Geoff Wahl
Terms and Licenses:
MTA

Comments:

Two sequence differences from consensus sequence (D26G and V119I) were detected in Addgene sequencing. This clone is as originally published and isolated.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Kanda et al (Curr Biol. 1998 Mar 26. 8(7):377-85. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11680" in your Materials and Methods section.