Addgene - H2B-Venus Plasmid Data

Plasmid 20971: H2B-Venus

Gene/insert name:
Histone cluster 1, H2bb
Alt name:
H2B.1
Insert size:
400
Species:
H. sapiens (human)
GenBank ID:
NM_021062
Entrez Gene:
HIST1H2BB (H2B.1, H2B/f, H2BFF)
Fusion protein or tag:
Venus
Terminal:
C terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
Sp6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Robert Benezra
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: High levels of Id1 expression define B1 type adult neural stem cells. Nam et al (Cell Stem Cell. 2009 Nov 6. 5(5):515-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20971" in your Materials and Methods section.