Addgene - pIRESneo-EGFP-alpha Tubulin Plasmid Data

Plasmid 12298: pIRESneo-EGFP-alpha Tubulin

Gene/insert name:
EGFP-alpha Tubulin
Alt name:
EGFP-Tubulin
Alt name:
k-alpha-1
Insert size:
2100
Species:
H. sapiens (human)
Entrez Gene:
TUBA1B (K-ALPHA-1)
Fusion protein or tag:
EGFP
Terminal:
N terminal on insert
Vector backbone:
pIRESneo2
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5300
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Patricia Wadsworth
Terms and Licenses:
MTA

Comments:

EGFP-Tubulin came from pEGFP-alpha Tubulin from Clontech

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin. Rusan et al (Mol Biol Cell. 2001 Apr . 12(4):971-80. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12298" in your Materials and Methods section.