Addgene - pCAG-mGFP-Actin Plasmid Data

Plasmid 21948: pCAG-mGFP-Actin

Gene/insert name:
betaActin
Alt name:
ACTB
Insert size:
1128
Species:
H. sapiens (human)
Entrez Gene:
ACTB (BRWS1, PS1TP5BP1)
Fusion protein or tag:
mEGFP
Terminal:
N terminal on insert
Vector backbone:
pCAG
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5802
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
gaccccaacgagaagcgcg List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Ryohei Yasuda
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Highly sensitive and quantitative FRET-FLIM imaging in single dendritic spines using improved non-radiative YFP. Murakoshi et al (Brain Cell Biol. 2008 Aug . 36(1-4):31-42. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21948" in your Materials and Methods section.