pReadthroughUAG
(Plasmid
#11511)
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Depositing Lab
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Depositing OrganizationMassachusetts Institute of Technology
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11511 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 11511-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 11511-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRL-TK
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4045
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFirefly luciferase, UAG stop codon, Renilla luciferase, and 3' UTR short RNA binding sites
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site see comments (unknown if destroyed)
- 3′ cloning site see comments (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The stop codon of the Firefly luciferase ORF from pGL3 was replaced with the stop codon UAG. This construct contains the Firefely ORF fused in frame to Renilla with 18 bp distance between the stop codon and the first ATG within the Renilla ORF. In addition, six CXCR4 short RNA binding sites are present.
DNA (Catalog # 11511-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 11511-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pReadthroughUAG was a gift from Phil Sharp (Addgene plasmid # 11511 ; http://n2t.net/addgene:11511 ; RRID:Addgene_11511) -
For your References section:
Short RNAs Repress Translation after Initiation in Mammalian Cells. Petersen CP, Bordeleau ME, Pelletier J, Sharp PA. Mol Cell. 2006 Feb 17. 21(4):533-42. 10.1016/j.molcel.2006.01.031 PubMed 16483934
Map uploaded by the depositor.
