pGM190oriT
(Plasmid #115679)

• Purpose: E. coli-Streptomyces shuttle vector for gene expression
• Depositing Lab: Günther Muth
• Publication: E.coli-Streptomyces shuttle vectors (unpublished)

Backbone

• Vector backbone: pSG5
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: oriT, aphII, tsr
• Species: Streptomyces
• Promoter: tipA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • pGM190-oriT.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mobilizable, stable replicating expression vector for Streptomyces, thiostrepton inducible promoter, aphII, tsr