Addgene - pcDNA3 merlin 1-595 Plasmid Data

Plasmid 11623: pcDNA3 merlin 1-595

Gene/insert name:
merlin
Alt name:
NF2
Alt name:
isoform 1
Insert size:
1785
Species:
H. sapiens (human)
Entrez Gene:
NF2 (ACN, BANF, SCH)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3/5'F
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Vijaya Ramesh
Terms and Licenses:
MTA

Comments:

Named pcDNA3/5'F-FLiso1 (aa 1-595) in reference.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Analysis of molecular domains of epitope-tagged merlin isoforms in Cos-7 cells and primary rat Schwann cells. Xu et al (Exp Cell Res. 1998 Jan 10. 238(1):231-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11623" in your Materials and Methods section.