pGEX2T merlin 1-595
(Plasmid #11629)

• Depositing Lab: Vijaya Ramesh
• Publication: Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-42.

Backbone

• Vector backbone: pGEX2T
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4950
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: merlin
• Alt name: NF2
• Alt name: isoform 1
• Species: H. sapiens (human)
• Insert Size (bp): 1785
• Entrez Gene: NF2 (a.k.a. ACN, BANF, SCH, SWNV, merlin-1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The merlin insert in this plasmid contains A190V and G302E mutations compared to the closest match human Merlin reference sequence (NP_000259.1). The depositing laboratory states that these mutations should not affect merlin function, but cautions that it may be necessary to test merlin function if performing structure function experiments.