Addgene - GAL-CDC12(WT) Plasmid Data

Plasmid 1165: GAL-CDC12(WT)

Gene/insert name:
CDC12
Insert size:
1318
Species:
S. cerevisiae (budding yeast)
GenBank ID:
X82498
Entrez Gene:
CDC12 (CLA10, PSL7)
Vector backbone:
pRS316:GAL1-10
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5554
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Comments:

7059

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dominant gain-of-function mutations in Hsp104p reveal crucial roles for the middle region. Schirmer et al (Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1165" in your Materials and Methods section.

Price: $65.00

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This is commonly requested with

1166: GAL-CDC12(K351N)

1167: GAL-CDC12(E368Q)