Addgene - pGL3-p300 reporter Plasmid Data

Plasmid 11726: pGL3-p300 reporter

Gene/insert name:
p300 promoter and 5' UTR
Alt name:
p300
Insert size:
1533
Entrez Gene:
EP300 (RP1-85F18.1, KAT3B, RSTS2, p300)
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Vector backbone:
pGL3-Basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4818
Cloning site 5':
KpnI
Site destroyed during cloning:
Unknown
Cloning site 3':
HindIII
Site destroyed during cloning:
Unknown
5' sequencing primer:
RVprimer3 List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Eileen Adamson
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Coactivating factors p300 and CBP are transcriptionally crossregulated by Egr1 in prostate cells, leading to divergent responses. Yu et al (Mol Cell. 2004 Jul 2. 15(1):83-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11726" in your Materials and Methods section.