Addgene - pCMV5B-TGFbeta receptor I K232R Plasmid Data

Gene/insert name:
TGFbeta receptor I
Insert size:
1600
Species:
H. sapiens (human)
GenBank ID:
NM_004612
Entrez Gene:
TGFBR1 (RP11-96L7.1, AAT5, ACVRLK4, ALK-5, ALK5, LDS1A, LDS2A, MSSE, SKR4, TGFR-1)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
K232R: kinase dead
Vector backbone:
pCMV5B
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: GS domain mutations that constitutively activate T beta R-I, the downstream signaling component in the TGF-beta receptor complex. Wieser et al (EMBO J. 1995 May 15. 14(10):2199-208. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11763" in your Materials and Methods section.