Addgene - pCMV5B-TFGbeta receptor II K227R Plasmid Data

Plasmid 11762: pCMV5B-TFGbeta receptor II K227R

Gene/insert name:
TGFbeta receptor II
Insert size:
1800
Species:
H. sapiens (human)
GenBank ID:
NM_003242
Entrez Gene:
TGFBR2 (AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, TGFbeta-RII)
Fusion protein or tag:
His
Terminal:
N terminal on insert
Mutation:
K227R: kinase dead
Vector backbone:
pCMV5B
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
This plasmid was constructed at the Massague lab at MSKCC. Please acknowledge both the Wrana and Massague labs in your publication.
Sequence: View sequences (1)
Principal Investigator:
Joan Massague
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TGF beta signals through a heteromeric protein kinase receptor complex. Wrana et al (Cell. 1992 Dec 11. 71(6):1003-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11762" in your Materials and Methods section.