- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LucNrev (Common Sequencing Primers)
A luciferase reporter gene with the 3TP
promoter that contains three consecutive TPA response elements (TREs) and a portion of the plasminogen activator
inhibitor 1 (PAI-1) promoter region.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3TP-lux was a gift from Joan Massague & Jeff Wrana (Addgene plasmid # 11767)
For your References section:TGF beta signals through a heteromeric protein kinase receptor complex. Wrana JL, Attisano L, Carcamo J, Zentella A, Doody J, Laiho M, Wang XF, Massague J. Cell. 1992 Dec 11. 71(6):1003-14. 10.1016/0092-8674(92)90395-S PubMed 1333888
Map generated by Addgene from full sequence.