Addgene - pARE-lux Plasmid Data

Gene/insert name:
ARE
Vector backbone:
pSP72-CAT
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
LucNrev List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
This plasmid was constructed at the Massague lab at MSKCC. Please acknowledge both the Wrana and Massague labs in your publication.
Sequence: View sequences (1)
Principal Investigator:
Joan Massague
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Contains three copies of the ARE (activin response element) from the Xenopus Mix.2 promoter linked to a basic TATA box and a luciferase reporter gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TGF beta signals through a heteromeric protein kinase receptor complex. Wrana et al (Cell. 1992 Dec 11. 71(6):1003-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11768" in your Materials and Methods section.