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pARE-lux
(Plasmid #11768)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11768 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2-Basic
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was constructed at the Massague lab at MSKCC. Please acknowledge both the Wrana and Massague labs in your publication.
  • Terms and Licenses

Depositor Comments

Contains three copies of the ARE (activin response element) from the Xenopus Mix.2 promoter linked to a basic TATA box and a luciferase reporter gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pARE-lux was a gift from Joan Massague & Jeff Wrana (Addgene plasmid # 11768 ; http://n2t.net/addgene:11768 ; RRID:Addgene_11768)
  • For your References section:

    TGF beta signals through a heteromeric protein kinase receptor complex. Wrana JL, Attisano L, Carcamo J, Zentella A, Doody J, Laiho M, Wang XF, Massague J. Cell. 1992 Dec 11. 71(6):1003-14. 10.1016/0092-8674(92)90395-S PubMed 1333888