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pCXN2-mVIN
(Plasmid #11776)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11776 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCXN2
  • Backbone manufacturer
    Niwa et al, Gene, v108, 1991
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vinculin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3200
  • Entrez Gene
    Vcl (a.k.a. 9430097D22, AA571387, AI462105, AW545629)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See author map (unknown if destroyed)
  • 3′ cloning site See author map (unknown if destroyed)
  • 5′ sequencing primer See author map
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCXN2-mVIN was a gift from Eileen Adamson (Addgene plasmid # 11776 ; http://n2t.net/addgene:11776 ; RRID:Addgene_11776)
  • For your References section:

    Rescue of the mutant phenotype by reexpression of full-length vinculin in null F9 cells; effects on cell locomotion by domain deleted vinculin. Xu W, Coll JL, Adamson ED. J Cell Sci. 1998 Jun . 111 ( Pt 11)():1535-44. PubMed 9580561