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pET15b CnA CnB
(Plasmid #11787)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.


  • Gene/Insert name
    CnA CnB
  • Alt name
  • Alt name
    Ppp3ca (calcineurin A subunit alpha)
  • Alt name
    Ppp3r1 (calcineurin B, type I)
  • Species
    H. sapiens (human)
  • Entrez Gene
    PPP3R1 (a.k.a. CNB, CNB1, CALNB1)
  • Entrez Gene
    PPP3CA (a.k.a. CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b CnA CnB was a gift from Anjana Rao (Addgene plasmid # 11787)
  • For your References section:

    Overexpression and purification of human calcineurin alpha from Escherichia coli and assessment of catalytic functions of residues surrounding the binuclear metal center. Mondragon A, Griffith EC, Sun L, Xiong F, Armstrong C, Liu JO. Biochemistry. 1997 Apr 22. 36(16):4934-42. 10.1021/bi9631935 PubMed 9125515