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pET15b CnA CnB
(Plasmid #11787)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11787 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CnA CnB
  • Alt name
    calcineurin
  • Alt name
    Ppp3ca (calcineurin A subunit alpha)
  • Alt name
    Ppp3r1 (calcineurin B, type I)
  • Species
    H. sapiens (human)
  • Entrez Gene
    PPP3R1 (a.k.a. CALNB1, CNB, CNB1)
  • Entrez Gene
    PPP3CA (a.k.a. ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, DEE91, IECEE, IECEE1, PPP2B)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b CnA CnB was a gift from Anjana Rao (Addgene plasmid # 11787 ; http://n2t.net/addgene:11787 ; RRID:Addgene_11787)
  • For your References section:

    Overexpression and purification of human calcineurin alpha from Escherichia coli and assessment of catalytic functions of residues surrounding the binuclear metal center. Mondragon A, Griffith EC, Sun L, Xiong F, Armstrong C, Liu JO. Biochemistry. 1997 Apr 22. 36(16):4934-42. 10.1021/bi9631935 PubMed 9125515