Addgene - pET15b CnA CnB Plasmid Data

Plasmid 11787: pET15b CnA CnB

Gene/insert name:
CnA CnB
Alt name:
calcineurin
Alt name:
Ppp3ca
Alt name:
Ppp3r1
Species:
H. sapiens (human)
Entrez Gene:
PPP3R1 (CNB, CNB1, CALNB1)
PPP3CA (CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B)
Vector backbone:
pET15b
(Search Vector Database)
Backbone manufacturer:
EMD Biosciences
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5700
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
JO Liu, 1997, PMID 9125515.
Sequence: View sequences (1)
Principal Investigator:
Anjana Rao
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Overexpression and purification of human calcineurin alpha from Escherichia coli and assessment of catalytic functions of residues surrounding the binuclear metal center. Mondragon et al (Biochemistry. 1997 Apr 22. 36(16):4934-42. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11787" in your Materials and Methods section.