Purpose3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17870||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name3x NFAT binding sequence
Alt nameNF-AT site
Entrez GeneIL2 (a.k.a. IL-2, TCGF, lymphokine)
- Promoter IL-2 promoter
/ Fusion Proteins
- IL2 promoter (C terminal on insert)
- Luciferase (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer RVprimer3 (5'-CTAGCAAAATAGGCTGTCCC-3')
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
This plasmid has three copies of the NF-AT site cloned upstream of the minimal IL-2 promoter from -89 to +51.
The plasmid can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue including lymphocytes, neurons, osteoblasts, skin, endothelium, skeletal and heart muscle (Shaw et al Science 1988; Crabtree and Schreiber Cell 138,210, 2009).
Based on Addgene's full plasmid sequence, the NFAT binding sequence appears to be ACGCCTTCTGTATGAAACAGTTTTTCCTCC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-NFAT luciferase was a gift from Jerry Crabtree (Addgene plasmid # 17870 ; http://n2t.net/addgene:17870 ; RRID:Addgene_17870)
For your References section:Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25. 357(6380):695-7. 10.1038/357695a0 PubMed 1377362