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pGL3-NFAT luciferase
(Plasmid #17870)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17870 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3x NFAT binding sequence
  • Alt name
    NF-AT site
  • Entrez Gene
    IL2 (a.k.a. IL-2, TCGF, lymphokine)
  • Promoter IL-2 promoter
  • Tags / Fusion Proteins
    • IL2 promoter (C terminal on insert)
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer RVprimer3 (5'-CTAGCAAAATAGGCTGTCCC-3')
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid has three copies of the NF-AT site cloned upstream of the minimal IL-2 promoter from -89 to +51.

The plasmid can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue including lymphocytes, neurons, osteoblasts, skin, endothelium, skeletal and heart muscle (Shaw et al Science 1988; Crabtree and Schreiber Cell 138,210, 2009).

Based on Addgene's full plasmid sequence, the NFAT binding sequence appears to be ACGCCTTCTGTATGAAACAGTTTTTCCTCC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-NFAT luciferase was a gift from Jerry Crabtree (Addgene plasmid # 17870)
  • For your References section:

    Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25. 357(6380):695-7. 10.1038/357695a0 PubMed 1377362