Addgene - pGL3-NFAT luciferase Plasmid Data

Plasmid 17870: pGL3-NFAT luciferase

Gene/insert name:
3x NFAT binding sequence
Alt name:
NF-AT site
Fusion protein or tag:
IL2 promoter
Terminal:
C terminal on insert
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Vector backbone:
pGL3
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4800
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

This plasmid has three copies of the NF-AT site cloned upstream of the minimal IL-2 promoter from -89 to +51.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Clipstone et al (Nature. 1992 Jun 25. 357(6380):695-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17870" in your Materials and Methods section.