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Plasmid 11783: NFAT/AP-1 3x luciferase
  • NFAT/AP-1 3x

  • IL-2 promoter

  • 120

  • M. musculus (mouse)

  • Il2 (DN-144H19.3, Il-2)

  • luciferase

  • C terminal on backbone

  • pT81Luc
    (Search Vector Database)

  • ATCC

  • Bacterial Expression, Luciferase

  • HindIII

  • Yes

  • XhoI

  • No

  • LucNrev List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • DJ McKean (Hedin, et al., 1997, PMID 9548483)

  • View sequences (2)
  • Anjana Rao

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

Three tandem repeats of the NFAT/AP-1 binding site at approximately -287 of the murine IL-2 promotor. Sequence: 5'-AGCTTGGATCC AAGAGGAAAA TTTGTTTCAT ACAGAAGGCG TTAAGAGGAA AATTTGTTTC ATACAGAAGG CGTTAAGAGG AAAATTTGTT TCATACAGAA GGCGTTCAAG CTTGTCGAC-3

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian et al (EMBO J. 2000 Sep 1. 19(17):4783-95. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11783" in your Materials and Methods section.