Addgene - NFAT/AP-1 3x luciferase Plasmid Data

Plasmid 11783: NFAT/AP-1 3x luciferase

Gene/insert name:
NFAT/AP-1 3x
Alt name:
IL-2 promoter
Insert size:
120
Species:
M. musculus (mouse)
Entrez Gene:
Il2 (DN-144H19.3, Il-2)
Fusion protein or tag:
luciferase
Terminal:
C terminal on backbone
Vector backbone:
pT81Luc
(Search Vector Database)
Backbone manufacturer:
ATCC
Vector type:
Bacterial Expression, Luciferase
Cloning site 5':
HindIII
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
LucNrev List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
DJ McKean (Hedin, et al., 1997, PMID 9548483)
Sequence: View sequences (2)
Principal Investigator:
Anjana Rao
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Three tandem repeats of the NFAT/AP-1 binding site at approximately -287 of the murine IL-2 promotor. Sequence: 5'-AGCTTGGATCC AAGAGGAAAA TTTGTTTCAT ACAGAAGGCG TTAAGAGGAA AATTTGTTTC ATACAGAAGG CGTTAAGAGG AAAATTTGTT TCATACAGAA GGCGTTCAAG CTTGTCGAC-3

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian et al (EMBO J. 2000 Sep 1. 19(17):4783-95. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11783" in your Materials and Methods section.