NFAT/AP-1 3x luciferase
(Plasmid #11783)

Loading...
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pT81Luc
  • Backbone manufacturer
    ATCC
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    NFAT/AP-1 3x
  • Alt name
    IL-2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    120
  • Entrez Gene
    Il2 (a.k.a. DN-144H19.3, Il-2)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Three tandem repeats of the NFAT/AP-1 binding site at approximately -287 of the murine IL-2 promotor. Sequence: 5'-AGCTTGGATCC AAGAGGAAAA TTTGTTTCAT ACAGAAGGCG TTAAGAGGAA AATTTGTTTC ATACAGAAGG CGTTAAGAGG AAAATTTGTT TCATACAGAA GGCGTTCAAG CTTGTCGAC-3

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NFAT/AP-1 3x luciferase was a gift from Anjana Rao (Addgene plasmid # 11783)
  • For your References section:

    Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian F, Garcia-Rodriguez C, Rao A. EMBO J. 2000 Sep 1. 19(17):4783-95. 10.1093/emboj/19.17.4783 PubMed 10970869