NFAT/AP-1 3x luciferase
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert nameNFAT/AP-1 3x
Alt nameIL-2 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)120
Entrez GeneIl2 (a.k.a. Il-2)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer LucNrev (Common Sequencing Primers)
Three tandem repeats of the NFAT/AP-1 binding site at approximately -287 of the murine IL-2 promotor. Sequence: 5'-AGCTTGGATCC AAGAGGAAAA TTTGTTTCAT ACAGAAGGCG TTAAGAGGAA AATTTGTTTC ATACAGAAGG CGTTAAGAGG AAAATTTGTT TCATACAGAA GGCGTTCAAG CTTGTCGAC-3
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NFAT/AP-1 3x luciferase was a gift from Anjana Rao (Addgene plasmid # 11783)
For your References section:Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian F, Garcia-Rodriguez C, Rao A. EMBO J. 2000 Sep 1. 19(17):4783-95. 10.1093/emboj/19.17.4783 PubMed 10970869
Map generated by Addgene from plasmid data.