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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 12194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepT81Luc
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Backbone manufacturerATCC
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Vector typeBacterial Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIL-2 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)524
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Entrez GeneIl2 (a.k.a. IL, Il-2)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer LucNrev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDJ McKean (Hedin, et al., 1997, PMID 9548483)
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
Murine IL-2 promotor from -585 to -34 relative to the transcription start site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IL-2 promoter luciferase was a gift from Anjana Rao (Addgene plasmid # 12194 ; http://n2t.net/addgene:12194 ; RRID:Addgene_12194) -
For your References section:
Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian F, Garcia-Rodriguez C, Rao A. EMBO J. 2000 Sep 1. 19(17):4783-95. 10.1093/emboj/19.17.4783 PubMed 10970869