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IL-2 promoter luciferase
(Plasmid #12194)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12194 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IL-2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    524
  • Entrez Gene
    Il2 (a.k.a. Il-2)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Murine IL-2 promotor from -585 to -34 relative to the transcription start site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IL-2 promoter luciferase was a gift from Anjana Rao (Addgene plasmid # 12194 ; http://n2t.net/addgene:12194 ; RRID:Addgene_12194)
  • For your References section:

    Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian F, Garcia-Rodriguez C, Rao A. EMBO J. 2000 Sep 1. 19(17):4783-95. 10.1093/emboj/19.17.4783 PubMed 10970869