IL-2 promoter luciferase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12194||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIL-2 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)524
Entrez GeneIl2 (a.k.a. IL, Il-2)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer LucNrev (Common Sequencing Primers)
Murine IL-2 promotor from -585 to -34 relative to the transcription start site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:IL-2 promoter luciferase was a gift from Anjana Rao (Addgene plasmid # 12194 ; http://n2t.net/addgene:12194 ; RRID:Addgene_12194)
For your References section:Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian F, Garcia-Rodriguez C, Rao A. EMBO J. 2000 Sep 1. 19(17):4783-95. 10.1093/emboj/19.17.4783 PubMed 10970869