IL13 promoter luciferase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11784||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGL2 Basic
- Backbone size w/o insert (bp) 5600
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIL-13 promoter
SpeciesH. sapiens (human)
Insert Size (bp)980
Entrez GeneIL13 (a.k.a. IL-13, P600)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer LucNrev (Common Sequencing Primers)
Human IL-13 5' flank region spanning
-940 to +48.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:IL13 promoter luciferase was a gift from Anjana Rao (Addgene plasmid # 11784 ; http://n2t.net/addgene:11784 ; RRID:Addgene_11784)
For your References section:Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian F, Garcia-Rodriguez C, Rao A. EMBO J. 2000 Sep 1. 19(17):4783-95. 10.1093/emboj/19.17.4783 PubMed 10970869