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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonena
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namek3(L)
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Alt nameTNF-alpha promoter element
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Alt namekappa3 long
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SpeciesH. sapiens (human)
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Insert Size (bp)40
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Entrez GeneTNF (a.k.a. DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
-117 to -78 of TNF-alpha promoter
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
k3(long) luciferase was a gift from Anjana Rao (Addgene plasmid # 11110 ; http://n2t.net/addgene:11110 ; RRID:Addgene_11110) -
For your References section:
Tumor necrosis factor alpha gene regulation in activated T cells involves ATF-2/Jun and NFATp. Tsai EY, Jain J, Pesavento PA, Rao A, Goldfeld AE. Mol Cell Biol. 1996 Feb . 16(2):459-67. PubMed 8552071