|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24942||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5597
Growth in Bacteria
Gene/Insert nameIL-10 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)1602
Entrez GeneIl10 (a.k.a. CSIF, Il-10)
/ Fusion Protein
- Luciferase (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
There are 3 point mutations in the promoter, but they should not affect activity.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2B -1538/+64 was a gift from Stephen Smale (Addgene plasmid # 24942 ; http://n2t.net/addgene:24942 ; RRID:Addgene_24942)
For your References section:A prominent role for Sp1 during lipopolysaccharide-mediated induction of the IL-10 promoter in macrophages. Brightbill HD, Plevy SE, Modlin RL, Smale ST. J Immunol. 2000 Feb 15. 164(4):1940-51. PubMed 10657644