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pGL2B -158/+64
(Plasmid #24946)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24946 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2B
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5597
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IL-10 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    222
  • Entrez Gene
    Il10 (a.k.a. CSIF, Il-10)
  • Tag / Fusion Protein
    • Luciferase (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2B -158/+64 was a gift from Stephen Smale (Addgene plasmid # 24946 ; http://n2t.net/addgene:24946 ; RRID:Addgene_24946)
  • For your References section:

    A prominent role for Sp1 during lipopolysaccharide-mediated induction of the IL-10 promoter in macrophages. Brightbill HD, Plevy SE, Modlin RL, Smale ST. J Immunol. 2000 Feb 15. 164(4):1940-51. PubMed 10657644