3xAP1pGL3 (3xAP-1 in pGL3-basic)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40342||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Alt nameThree canonical AP-1 binding sites (TGACTCA)
MutationContains three canonical AP-1 binding sites (TGACTCA) upstream of a minimal promoter fragment containing a TATA box
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
The 3 AP-1 reporter contains three canonical AP-1 binding sites (TGACTCA) upstream of a minimal promoter fragment containing a TATA box in the luciferase reporter plasmid pGL3-basic.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342 ; http://n2t.net/addgene:40342 ; RRID:Addgene_40342)
For your References section:Repression of AP-1 function: a mechanism for the regulation of Blimp-1 expression and B lymphocyte differentiation by the B cell lymphoma-6 protooncogene. Vasanwala FH, Kusam S, Toney LM, Dent AL. J Immunol. 2002 Aug 15;169(4):1922-9. PubMed 12165517