Addgene - p416 103Q GPD Plasmid Data

Plasmid 1180: p416 103Q GPD

Gene/insert name:
htt 103Q
Insert size:
500
Species:
H. sapiens (human)
GenBank ID:
NM_002111
Entrez Gene:
HTT (HD, IT15)
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Vector backbone:
p416 GPD
(Search Vector Database)
Vector type:
Yeast Expression
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
M13R List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
URA3
Sequence: View sequences (1)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Comments:

N terminal region of Ht with polyQ repeat length of 103 fused to GFP.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins. Krobitsch et al (Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1589-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1180" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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