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Plasmid 1180: p416 103Q GPD
  • htt 103Q

  • 500

  • H. sapiens (human)

  • NM_002111

  • HTT (HD, IT15)

  • GFP

  • C terminal on insert

  • p416 GPD
    (Search Vector Database)

  • Yeast Expression

  • XhoI

  • No

  • XhoI

  • No

  • M13R List of Sequencing Primers

  • EGFP-N

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • URA3

  • View sequences (1)
  • Susan Lindquist

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

N terminal region of Ht with polyQ repeat length of 103 fused to GFP.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins. Krobitsch et al (Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1589-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1180" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only