pUPD3
(Plasmid #118043)

• Purpose: (Empty Backbone) GoldenBraid2.0 Universal Part Domesticator #3
• Depositing Lab: Koen Venken
• Publication: Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.

Backbone

• Vector backbone: pUPD
• Backbone manufacturer: Self made; Derived from pBluescriptSK
• Backbone size (bp): 2804
• Modifications to backbone: Chloramphenicol Resistance
• Vector type: Synthetic Biology ; Domestication of DNA parts for GoldenBraid asembly

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: taatacgactcactatagg
• 3′ sequencing primer: tctatagtgtcacctaaat

Resource Information

• Supplemental Documents:
  • pUPD3.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a GB2.0 domesticator vector. GBParts can be assembled in a BsmBI-ligation reaction. Any assembled Part can be released with BsaI for further cloning into Alpha level vectors.