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ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
(Plasmid #118412)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118412 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFB-AAV-mcs-shuttle_UIowa G0202
  • Backbone manufacturer
    University of Iowa
  • Backbone size w/o insert (bp) 7290
  • Total vector size (bp) 8943
  • Modifications to backbone
    Has both the Tn7/GmR components for making a baculovirus expressing AAV, as well as the AmpR components for use when producing plasmid in E.coli for use when making AAV in human HEK293(T) cells
  • Vector type
    Mammalian Expression, Insect Expression, AAV, Luciferase, Synthetic Biology
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Must check ITR integrity by restriction digest with AhdI, as well as with XmaI, to ensure the vector hasn't recombined following amplification.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly luciferase
  • Alt name
    FLuc
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1653
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TCAGACAGTGGTTCAAAG
  • 3′ sequencing primer CATTAAAGCAGCGTATCC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293 was a gift from Nicole Paulk (Addgene plasmid # 118412 ; http://n2t.net/addgene:118412 ; RRID:Addgene_118412)
  • For your References section:

    Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors. Rumachik NG, Malaker SA, Poweleit N, Maynard LH, Adams CM, Leib RD, Cirolia G, Thomas D, Stamnes S, Holt K, Sinn P, May AP, Paulk NK. Mol Ther Methods Clin Dev. 2020 May 22;18:98-118. doi: 10.1016/j.omtm.2020.05.018. eCollection 2020 Sep 11. 10.1016/j.omtm.2020.05.018 PubMed 32995354