Skip to main content
Holiday Schedule: Addgene will be closed December 24 - December 31. Order processing and shipping will resume on January 3, 2022. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pPAGFP-C1
(Plasmid #11910)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11910 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 11910-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PAGFP
  • Alt name
    photoactivatable green fluorescent protein
  • Species
    Aequorea victoria
  • Mutation
    These plasmids are essentially the same as the Clontech pEGFP-C1 version with four mutations (L64F/T65S/V163A/T203H). The PAGFP also contains an A206K mutation to disrupt dimerization

Cloning Information

Resource Information

Depositor Comments

The pPAGFP-C1 was not published. It was prepared by removing the PAGFP cDNA from pPAGFP-N1 with an AgeI/BsrGI restriction digest and subcloning it into a similarly digested pEYFP-C1 (Clontech). The pPAGFP-C1 was confirmed by sequencing. If you use this construct in future publications, use your best judgement for citation.

The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent.

The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.

Information for Cloning Grade DNA (Catalog # 11910-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPAGFP-C1 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 11910 ; http://n2t.net/addgene:11910 ; RRID:Addgene_11910)
  • For your References section:

    A photoactivatable GFP for selective photolabeling of proteins and cells. Patterson GH, Lippincott-Schwartz J. Science. 2002 Sep 13. 297(5588):1873-7. 10.1126/science.1074952 PubMed 12228718