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p2UGFP
(Plasmid #1195)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p2UGpd
  • Backbone size w/o insert (bp) 6016
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    GFP w/ stop codon
  • Species
    jellyfish
  • Insert Size (bp)
    720

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

2 micron yeast plasmid. GPD promoter. Map of empty vector included here.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2UGFP was a gift from Susan Lindquist (Addgene plasmid # 1195)
  • For your References section:

    Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino MM, Liu JJ, Glover JR, Lindquist S. Science 1996 Aug 2;273(5275):622-6. 10.1126/science.273.5275.622 PubMed 8662547