Addgene - p2UGFP Plasmid Data

Plasmid 1195: p2UGFP

Gene/insert name:
GFP w/ stop codon
Insert size:
720
Species:
jellyfish
Vector backbone:
p2UGpd
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
6016
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
Yes
5' sequencing primer:
M13 Reverse List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Comments:

2 micron yeast plasmid. GPD promoter. Map of empty vector included here.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino et al (Science 1996 Aug 2;273(5275):622-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1195" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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