- Backbone size w/o insert (bp) 6016
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert nameGFP w/ stop codon
Insert Size (bp)720
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SpeI (destroyed during cloning)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
2 micron yeast plasmid. GPD promoter. Map of empty vector included here.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2UGFP was a gift from Susan Lindquist (Addgene plasmid # 1195)
For your References section:Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino MM, Liu JJ, Glover JR, Lindquist S. Science 1996 Aug 2;273(5275):622-6. 10.1126/science.273.5275.622 PubMed 8662547
Map uploaded by the depositor.