PurposeLevel 0 Part. Insertion site
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||119643||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2247
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name7942 NS2 Up Flank
Insert Size (bp)800
Mutationbp 422 A to T, bp 423 A to T, bp 663 inserted G. Deletion of nt 664 in NS2 Up Flank.
- Cloning method Restriction Enzyme
- 5′ cloning site BpiI (destroyed during cloning)
- 3′ cloning site BpiI (destroyed during cloning)
- 5′ sequencing primer ttgagtgagctgataccgct
- 3′ sequencing primer GTCTCATGAGCGGATACATATTTGAATG (Common Sequencing Primers)
Addgene QC NGS identified a sequence discrepancy in the plasmid insert. The depositing lab has confirmed that this should not affect plasmid function.
Synechoccoccus elongatus PCC 7942, sequence upstream of Synpcc7942_0085.. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pC0.175 was a gift from Alistair McCormick (Addgene plasmid # 119643 ; http://n2t.net/addgene:119643 ; RRID:Addgene_119643)
For your References section:CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria Based on the Plant MoClo Syntax. Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick AJ. Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub 2019 Feb 28. 10.1104/pp.18.01401 PubMed 30819783