CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria Based on the Plant MoClo Syntax.
Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick AJ
Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub 2019 Feb 28. PubMed Article
Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick AJ
Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub 2019 Feb 28. PubMed Article
A subset of these plasmids is included in the CyanoGate Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119555 | pCAT.011 | Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector. |
| 119556 | pCAT.334 | Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector. |
| 119557 | pCA0.001 | Level 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screening |
| 119558 | pCAT.015 | Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector. |
| 119559 | pCAT.000 | Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori. |
| 119560 | pCA0.002 | Level 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori. |
| 119561 | pC0.049 | Level 0 part. Promoter |
| 119562 | pC0.034 | Level 0 part. Promoter |
| 119563 | pC0.041 | Level 0 part. Promoter |
| 119564 | pC.059 | Level 0 part. Promoter |
| 119565 | pC0.057 | Level 0 part. Promoter |
| 119566 | pC0.055 | Level 0 part. Promoter |
| 119567 | pC0.005 | Level 0 part. Promoter |
| 119568 | pC0.050 | Level 0 part. Promoter |
| 119569 | pC0.051 | Level 0 part. Promoter |
| 119570 | pC0.052 | Level 0 part. Promoter |
| 119571 | pC0.053 | Level 0 part. Promoter |
| 119572 | pC0.054 | Level 0 part. Promoter |
| 119573 | pC0.056 | Level 0 part. Promoter |
| 119574 | pC0.093 | Level 0 part. Promoter |
| 119575 | pC0.030 | Level 0 part. Promoter |
| 119576 | pC0.031 | Level 0 part. Promoter |
| 119577 | pC0.032 | Level 0 part. Promoter |
| 119578 | pC0.033 | Level 0 part. Promoter |
| 119579 | pC0.035 | Level 0 part. Promoter |
| 119580 | pC0.036 | Level 0 part. Promoter |
| 119581 | pC0.037 | Level 0 part. Promoter |
| 119582 | pC0.038 | Level 0 part. Promoter |
| 119583 | pC0.039 | Level 0 part. Promoter |
| 119584 | pC0.040 | Level 0 part. Promoter |
| 119586 | pC0.043 | Level 0 part. Promoter |
| 119587 | pC0.044 | Level 0 part. Promoter |
| 119588 | pC0.045 | Level 0 part. Promoter |
| 119589 | pC0.046 | Level 0 part. Promoter |
| 119590 | pC0.047 | Level 0 part. Promoter |
| 119591 | pC0.048 | Level 0 part. Promoter |
| 119592 | pC0.083 | Level 0 part. Promoter |
| 119593 | pC0.084 | Level 0 part. Promoter |
| 119594 | pC0.085 | Level 0 part. Promoter |
| 119595 | pC0.088 | Level 0 part. Promoter |
| 119596 | pC0.089 | Level 0 part. Promoter |
| 119597 | pC0.090 | Level 0 part. Promoter |
| 119598 | pC0.091 | Level 0 part. Promoter |
| 119599 | pC0.092 | Level 0 part. Promoter |
| 119600 | pC0.285 | Level 0 part. Promoter |
| 119601 | pC.264 | Level 0 part. Promoter |
| 119602 | pC.060 | Level 0 part. Promoter |
| 119603 | pC.061 | Level 0 part. Promoter |
| 119604 | pC0.203 | Level 0 part. TSS promoter |
| 119605 | pC0.208 | Level 0 part. TSS promoter |
| 119606 | pC0.215 | Level 0 part. TSS promoter |
| 119607 | pC0.219 | Level 0 part. TSS promoter |
| 119608 | pC0.220 | Level 0 part. TSS promoter |
| 119609 | pC0.078 | Level 0 part. Terminator |
| 119610 | pC0.081 | Level 0 part. Terminator |
| 119611 | pC0.082 | Level 0 part. Terminator |
| 119612 | pC0.116 | Level 0 part. Linker |
| 119613 | pC0.117 | Level 0 part. Linker |
| 119614 | pC0.118 | Level 0 part. Linker |
| 119615 | pC0.119 | Level 0 part. Linker |
| 119616 | pC0.120 | Level 0 part. Linker |
| 119617 | pC0.121 | Level 0 part. Linker |
| 119618 | pC0.008 | Level 0 Part. CDS |
| 119619 | pC0.009 | Level 0 Part. CDS |
| 119620 | pC0.161 | Level 0 Part. CDS |
| 119621 | pC0.017 | Level 0 Part. CDS |
| 119622 | pC0.018 | Level 0 Part. CDS |
| 119623 | pC0.026 | Level 0 Part. CDS |
| 119624 | pC0.027 | Level 0 Part. CDS |
| 119625 | pC0.028 | Level 0 Part. CDS |
| 119626 | pC0.029 | Level 0 Part. CDS |
| 119627 | pC0.286 | Level 0 Part. CDS |
| 119628 | pC0.265 | Level 0 Part. CDS |
| 119629 | pC0.122 | Level 0 Part. CDS |
| 119630 | pC0.123 | Level 0 Part. CDS |
| 119631 | pC0.025 | Level 0 Part. Insertion site |
| 119632 | pC0.024 | Level 0 Part. Insertion site |
| 119633 | pC0.166 | Level 0 Part. Insertion site |
| 119634 | pC0.165 | Level 0 Part. Insertion site |
| 119635 | pC0.168 | Level 0 Part. Insertion site |
| 119636 | pC0.167 | Level 0 Part. Insertion site |
| 119637 | pC0.169 | Level 0 Part. Insertion site |
| 119638 | pC0.170 | Level 0 Part. Insertion site |
| 119639 | pC0.171 | Level 0 Part. Insertion site |
| 119640 | pC0.172 | Level 0 Part. Insertion site |
| 119641 | pC0.174 | Level 0 Part. Insertion site |
| 119642 | pC0.173 | Level 0 Part. Insertion site |
| 119643 | pC0.175 | Level 0 Part. Insertion site |
| 119644 | pC0.176 | Level 0 Part. Insertion site |
| 119645 | pC0.178 | Level 0 Part. Insertion site |
| 119646 | pC0.177 | Level 0 Part. Insertion site |
| 119647 | pC0.281 | Level 0 Part. Insertion site |
| 119648 | pC0.282 | Level 0 Part. Insertion site |
| 119649 | pC0.283 | Level 0 Part. Insertion site |
| 119650 | pC0.284 | Level 0 Part. Insertion site |
| 119651 | pC0.062 | Level 0 part. Terminator for luxICDABEG operon from Vibrio fischeri. |
| 119652 | pC0.070 | Level 0 part. Terminator, Bi-directional terminator for yciA/tonA genes from E. coli. |
| 119655 | pC0.064 | Level 0 part. Terminator from hupB gene from E. coli. |
| 119656 | pC0.077 | Level 0 part. Terminator from rpoH gene from E. coli. |
| 119657 | pC0.072 | Level 0 part. Terminator from hyaABCDEF operon from E. coli. |
| 119658 | pC0.068 | Level 0 part. Terminator for clpPX gene from E. coli. |
| 119659 | pC0.065 | Level 0 part. Terminator araBAD operon from E. coli. |
| 119660 | pC0.073 | Level 0 part. Terminator from rplJL-rpoBC,rplKAJL-rpoBC,rpoBC gene cluster from E. coli. |
| 119661 | pC0.069 | Level 0 part. Terminator from arcA gene from E. coli. |
| 119662 | pC0.071 | Level 0 part. Terminator from tatABCD operon from E. coli. |
| 119663 | pC0.074 | Level 0 part. Terminator from Cysteine synthase A gene (cysK) from E. coli. |
| 119664 | pC0.063 | Level 0 part. Terminator derived from Biobricks plasmid pSB1AK3. |
| 119665 | pC0.080 | Level 0 part. Terminator for the cpcG1 gene Synechocystis sp. PCC 6803. |
| 119666 | pC0.066 | Level 0 part. Terminator from pheA gene from E. coli. |
| 119667 | pC0.079 | Level 0 part. Terminator for the psbA2 gene Synechocystis sp. PCC 6803. |
| 119669 | pC0.004 | Level 0 part. Promoter |
| 119670 | pC0.006 | Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated. |
| 119671 | pC0.007 | Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated. |