pCS2(+)-zGrad
(Plasmid #119716)

• Purpose: Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish
• Depositing Lab: Holger Knaut
• Publication: Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10.7554/eLife.43125.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	119716	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pCS2
  (Search Vector Database)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: zGrad
• Alt name: fusion of F-box from fbxw11b and anti-GFP lama single domain antibody vhhGFP4
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1005

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • 1124-pCS2-Fbxw11b-vhhGFP4 for addgene.gbk
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry