Addgene - Alpha 5 integrin-GFP Plasmid Data

Gene/insert name:
alpha 5 integrin
Alt name:
Itga5
Insert size:
3200
Species:
H. sapiens (human)
GenBank ID:
X06256
Entrez Gene:
ITGA5 (CD49e, FNRA, VLA5A)
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N3
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
Kpn1
Site destroyed during cloning:
No
Cloning site 3':
Kpn1
Site destroyed during cloning:
No
5' sequencing primer:
CMV immediate early gene forward primer List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Dr. Louis Reichardt UCSF
Sequence: View sequences (3)
Principal Investigator:
Rick Horwitz
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. Laukaitis et al (J Cell Biol. 2001 Jun 25. 153(7):1427-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15238" in your Materials and Methods section.