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pRK5 beta1
(Plasmid #16042)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16042 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta1
  • Alt name
    beta 1 integrin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3600
  • Entrez Gene
    ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the beta-1 integrin ORF has the following amino acid changes - C17G, M170T and N227S. Addgene is not aware of the impact of these mutations on the plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 beta1 was a gift from Filippo Giancotti (Addgene plasmid # 16042 ; http://n2t.net/addgene:16042 ; RRID:Addgene_16042)
  • For your References section:

    Elevated levels of the alpha 5 beta 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells. Giancotti FG, Ruoslahti E. Cell. 1990 Mar 9. 60(5):849-59. 10.1016/0092-8674(90)90098-Y PubMed 2155708