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pRK5 alpha6
(Plasmid #16036)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16036 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha6
  • Alt name
    alpha 6 integrin
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_000210.2
  • Entrez Gene
    ITGA6 (a.k.a. CD49f, ITGA6B, VLA-6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene's sequencing results indicate that this plasmid contains alpha-6 integrin isoform b.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 alpha6 was a gift from Filippo Giancotti (Addgene plasmid # 16036 ; http://n2t.net/addgene:16036 ; RRID:Addgene_16036)
  • For your References section:

    Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of hemidesmosomes. Dans M, Gagnoux-Palacios L, Blaikie P, Klein S, Mariotti A, Giancotti FG. J Biol Chem. 2001 Jan 12. 276(2):1494-502. 10.1074/jbc.M008663200 PubMed 11044453