|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16036||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namealpha 6 integrin
SpeciesH. sapiens (human)
Entrez GeneITGA6 (a.k.a. CD49f, ITGA6B, VLA-6)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Addgene's sequencing results indicate that this plasmid contains alpha-6 integrin isoform b.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5 alpha6 was a gift from Filippo Giancotti (Addgene plasmid # 16036 ; http://n2t.net/addgene:16036 ; RRID:Addgene_16036)
For your References section:Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of hemidesmosomes. Dans M, Gagnoux-Palacios L, Blaikie P, Klein S, Mariotti A, Giancotti FG. J Biol Chem. 2001 Jan 12. 276(2):1494-502. 10.1074/jbc.M008663200 PubMed 11044453