|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16039||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1(-)/Myc-His B
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt namebeta 4 integrin
SpeciesH. sapiens (human)
Insert Size (bp)5600
Entrez GeneITGB4 (a.k.a. CD104, GP150)
/ Fusion Proteins
- Myc (C terminal on backbone)
- His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Please note: The plasmid contains a S1694T mutation compared to NCBI reference sequence AAC51632.1. This mutation is a natural variant which is not expected to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1/Myc-His beta4 was a gift from Filippo Giancotti (Addgene plasmid # 16039 ; http://n2t.net/addgene:16039 ; RRID:Addgene_16039)
For your References section:Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of hemidesmosomes. Dans M, Gagnoux-Palacios L, Blaikie P, Klein S, Mariotti A, Giancotti FG. J Biol Chem. 2001 Jan 12. 276(2):1494-502. 10.1074/jbc.M008663200 PubMed 11044453