PurposeGABAA receptor expression (chimeric rat delta subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||119731||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe gamma 2 gene was originally cloned in Peter Seeburg’s lab in Heidelberg. The rat delta was originally from Dr. Robert Macdonald at Vanderbilt.
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Myc-delta/gamma2SIL was a gift from Angel de Blas (Addgene plasmid # 119731 ; http://n2t.net/addgene:119731 ; RRID:Addgene_119731)