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Myc-delta/gamma2SIL
(Plasmid #119731)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119731 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GABRD/GABRG2
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Gabrg2
  • Entrez Gene
    Gabrd (a.k.a. GABAA-RD)
  • Tag / Fusion Protein
    • cMyc epitope EQKLISEEDL inserted between fourth and fifth amino acids of mature protein after signal peptide is cleaved (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gamma 2 gene was originally cloned in Peter Seeburg’s lab in Heidelberg. The rat delta was originally from Dr. Robert Macdonald at Vanderbilt.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-delta/gamma2SIL was a gift from Angel de Blas (Addgene plasmid # 119731 ; http://n2t.net/addgene:119731 ; RRID:Addgene_119731)