Addgene - pCGN-SRF Plasmid Data

Plasmid 11977: pCGN-SRF

Gene/insert name:
SRF
Species:
H. sapiens (human)
Entrez Gene:
SRF (MCM1)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
pCGN
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
7500
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
Bglob-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Ron Prywes
Terms and Licenses:
MTA

Comments:

See author's map and paper for cloning details. pCGN: expression is driven by CMV promoter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of transcriptional activation and inhibitory domains in serum response factor (SRF) by using GAL4-SRF constructs. Johansen et al (Mol Cell Biol. 1993 Aug . 13(8):4640-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11977" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

14930: pCMV5 Smad2-HA