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pFOS WT-GL3
(Plasmid #11983)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11983 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-promoter modified
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-fos promoter
  • Alt name
    c-fos
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    465
  • Entrez Gene
    Fos (a.k.a. D12Rfj1, c-fos, cFos)
  • Tag / Fusion Protein
    • Luc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See author map (destroyed during cloning)
  • 3′ cloning site See author map (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See author's map for cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFOS WT-GL3 was a gift from Ron Prywes (Addgene plasmid # 11983 ; http://n2t.net/addgene:11983 ; RRID:Addgene_11983)
  • For your References section:

    Megakaryoblastic leukemia 1, a potent transcriptional coactivator for serum response factor (SRF), is required for serum induction of SRF target genes. Cen B, Selvaraj A, Burgess RC, Hitzler JK, Ma Z, Morris SW, Prywes R. Mol Cell Biol. 2003 Sep . 23(18):6597-608. 10.1128/MCB.23.18.6597-6608.2003 PubMed 12944485