PurposeReporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sites
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11982||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namec-jun promoter
SpeciesM. musculus (mouse)
Insert Size (bp)375
Entrez GeneJun (a.k.a. AP-1, Junc, c-ju, c-jun)
/ Fusion Protein
- Luc (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer NA (Common Sequencing Primers)
Plasmids pJC6GL3, pJSXGL3, pJTXGL3, and pJSTXGL3 contain positions -225 to +150 of the murine c-jun promoter. pJSXGL3, pJTXGL3, and pJSTXGL3 contain mutations at the MEF2 site, ATF (AP1-like) site, and both sites, respectively.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJSTX-GL3 was a gift from Ron Prywes (Addgene plasmid # 11982 ; http://n2t.net/addgene:11982 ; RRID:Addgene_11982)
For your References section:Epidermal growth factor induction of the c-jun promoter by a Rac pathway. Clarke N, Arenzana N, Hai T, Minden A, Prywes R. Mol Cell Biol. 1998 Feb . 18(2):1065-73. PubMed 9448004