pGL
(Plasmid #119952)

• Purpose: Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.
• Depositing Lab: Nigel Scrutton
• Publication: Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398.

Backbone

• Vector backbone: pBbB2a-GFP
• Backbone manufacturer: Lee, Taek Soon, et al. "BglBrick vectors and datasheets: a synthetic biology platform for gene expression." Journal of biologica
• Total vector size (bp): 5880
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: gpps
• Species: Abies grandis
• Mutation: N-terminal truncation of 85 amino acid residues (signal sequence)

Gene/Insert 2

• Gene/Insert name: limS
• Species: Mentha spicata
• Mutation: N-terminal truncation of 56 amino acid residues (signal sequence)

Resource Information

• Supplemental Documents:
  • pGL.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry