pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
(Plasmid #35152)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
  • Species
    S. cerevisiae (budding yeast)
  • Promoter LacUV5, pTrc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1, BglII (not destroyed)
  • 3′ cloning site BamH1, Xho1 (not destroyed)
  • 5′ sequencing primer gatcgtttaggcacccca
  • 3′ sequencing primer ATTAATAAGATGATC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5c-MevT(CO)-T1-MBIS(CO, ispA) was a gift from Jay Keasling & Taek_Soon Lee (Addgene plasmid # 35152)
  • For your References section:

    Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. 10.1038/ncomms1494 PubMed 21952217