Addgene - pBbA5c-MevT(CO)-T1-MBIS(CO, ispA) Plasmid Data

Plasmid 35152: pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Gene/insert name:
AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
Species:
S. cerevisiae (budding yeast)
Vector backbone:
pBbA5c
(Search Vector Database)
Vector type:
Bacterial Expression
Promoter:
LacUV5, pTrc
Cloning site 5':
EcoR1, BglII
Site destroyed during cloning:
No
Cloning site 3':
BamH1, Xho1
Site destroyed during cloning:
No
5' sequencing primer:
gatcgtttaggcacccca List of Sequencing Primers
3' sequencing primer:
ATTAATAAGATGATC
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (5)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Jay Keasling
Principal Investigator:
Taek_Soon Lee
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya et al (Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35152" in your Materials and Methods section.