Addgene - pTrcAgBIS (CO) Plasmid Data

Plasmid 35153: pTrcAgBIS (CO)

Gene/insert name:
Bisabolene Synthase
Species:
Abies grandis
Vector backbone:
pTrc99a
(Search Vector Database)
Vector type:
Bacterial Expression
Promoter:
pTrc
Cloning site 5':
Nco1
Site destroyed during cloning:
No
Cloning site 3':
Xba1, Sma1, BamH1, Xma1, Sal1, Pst1, Sph1, HindIII
Site destroyed during cloning:
No
5' sequencing primer:
aggtcgtaaatcact List of Sequencing Primers
3' sequencing primer:
tgagagaagattttcagcc
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Jay Keasling
Principal Investigator:
Taek_Soon Lee
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya et al (Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35153" in your Materials and Methods section.