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pGL3-Basic-OPN-1200
(Plasmid #11996)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11996 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Osteopontin
  • Alt name
    OPN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Mutation
    The following construct contains the OPN promoter, which drives the expression of luciferase.
  • GenBank ID
    NT_016354
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nhe1 (not destroyed)
  • 3′ cloning site xho1 (not destroyed)
  • 5′ sequencing primer rvprimer3
  • 3′ sequencing primer glprimer2
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Basic-OPN-1200 was a gift from Steven Johnson (Addgene plasmid # 11996 ; http://n2t.net/addgene:11996 ; RRID:Addgene_11996)