pAM4889 (pCV0001)
(Plasmid #120079)

• Purpose: Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.
• Depositing Lab: Susan Golden
• Publication: Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku673.

Backbone

• Vector backbone: RSF1010Y25F_aadA_PconII-LTRBS-GFPmut2
• Backbone size w/o insert (bp): 8069
• Modifications to backbone: Plasmid assembled from 3 CYANO-VECTOR modular devices: pCVD047 / CV-RSF1010Y25F: The device carries a broad host range replicon derived from RSF1010. A point mutation in mobA prevent self-mobilization. pCVD002 / CV-aadA: The device confers spectinomycin and streptomycin (SpSm) resistance. The stop codon was erased by mistake, another stop codon is however located 15 nt downstream in the transcriptional terminator. This device works perfectly well. pCVD030 / CV-PconII-LTRBS-GFPmut2: Promoter/reporter device where GFPmut2 is driven by a PconII promoter. The RBS and flanking sequences are from the pENTR/SD/D-Topo vector (Life Technologies).
• Vector type: Other

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin and Streptomycin, 50 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
• Species: Broad range of bacteria
• Mutation: mobA Y25F

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pAM4889 (pcv0001).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry