Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pUbiGUSPlus
(Plasmid #64402)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64402 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin (Ubi-1) promoter
  • Species
    maize
  • Promoter Ubi-1
  • Tag / Fusion Protein
    • GUSPlus reporter

Cloning Information

Resource Information

Depositor Comments

Plant ubiquitin (Ubi-1) promoter (with untranslated region intron) driving expression of a synthetic, optimised GUSPlus gene (CAMBIA) followed by an NOS terminator. The GUSPlus gene is used as a reporter gene for quantitative promoter expression in plant tissues.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUbiGUSPlus was a gift from Claudia Vickers (Addgene plasmid # 64402 ; http://n2t.net/addgene:64402 ; RRID:Addgene_64402)
  • For your References section:

    A synthetic xylanase as a novel reporter in plants. Vickers CE, Xue GP, Gresshoff PM. Plant Cell Rep. 2003 Sep;22(2):135-40. Epub 2003 Jul 4. 10.1007/s00299-003-0667-9 PubMed 12845475