PurposeUbiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64402||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typePlant Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameubiquitin (Ubi-1) promoter
- Promoter Ubi-1
/ Fusion Protein
- GUSPlus reporter
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Plant ubiquitin (Ubi-1) promoter (with untranslated region intron) driving expression of a synthetic, optimised GUSPlus gene (CAMBIA) followed by an NOS terminator. The GUSPlus gene is used as a reporter gene for quantitative promoter expression in plant tissues.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUbiGUSPlus was a gift from Claudia Vickers (Addgene plasmid # 64402 ; http://n2t.net/addgene:64402 ; RRID:Addgene_64402)
For your References section:A synthetic xylanase as a novel reporter in plants. Vickers CE, Xue GP, Gresshoff PM. Plant Cell Rep. 2003 Sep;22(2):135-40. Epub 2003 Jul 4. 10.1007/s00299-003-0667-9 PubMed 12845475