Purposebinary plant vector expressing GFP and GUS, both driven by a 35S promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64401||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typePlant Expression ; binary vector for plant transformation
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Promoter 35S
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-R (Common Sequencing Primers)
- Promoter 35S
/ Fusion Protein
- His (C terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
GenBank record number for the full plasmid seqeunce: EF546437.1
The 35S:GFP:NOS expression cassette from p35SsGFP was cloned into pCAMBIA1305.1 upstream and in the opposite orientation to the 35S:GUSPlus:NOS cassette. Expression of both reporter genes benefits from the enhancer elements found in the two 35S promoters.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGFPGUSPlus was a gift from Claudia Vickers (Addgene plasmid # 64401 ; http://n2t.net/addgene:64401 ; RRID:Addgene_64401)
For your References section:pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in plants. Vickers CE, Schenk PM, Li D, Mullineaux PM, Gresshoff PM. Biotechnol Lett. 2007 Nov;29(11):1793-6. Epub 2007 Aug 9. 10.1007/s10529-007-9467-6 PubMed 17687623