Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
(Plasmid #61628)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61628 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGreenII
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 9692
  • Vector type
    Plant Expression
  • Selectable markers
    Methothrexate

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    ntdTomato
  • Species
    Synthetic
  • Insert Size (bp)
    1518
  • GenBank ID
    KJ081243.1
  • Promoter Auxin-dependent DR5v2 promoter containing 9 reverse TGTCGG sites and -46 35S promoter

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer M13_pUC_fwd
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    n2xEGFP
  • Species
    Synthetic
  • Insert Size (bp)
    2100
  • Mutation
    2 copies of EGFP with N-terminal SV40 NLS
  • GenBank ID
    AF302837
  • Promoter Auxin-dependent DR5 promoter containing 9 reverse TGTCTC sites and -46 35S promoter

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer dsRed1_C_primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that Addgene NGS results identified as stop codon after the second EGFP ORF. This stop codon was present in the original, published version of the plasmid, such that the insert is n2GFP rather than n3GFP. The plasmid functions as described in the associated publication.

The diagnostic digest indicates this plasmid may be bigger than the assembled sequence suggests.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGreenIIM DR5v2-ntdTomato/DR5-n3GFP was a gift from Dolf Weijers (Addgene plasmid # 61628 ; http://n2t.net/addgene:61628 ; RRID:Addgene_61628)
  • For your References section:

    Reporters for sensitive and quantitative measurement of auxin response. Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2. 10.1038/nmeth.3279 PubMed 25643149